The Sitecore instance that connects to the xconnect website in your local machine try to follow the below options to solve the issue.
A ) In powershell administrator mode try to run below command to check how many certificate are in trusted root folder .
PS C:\WINDOWS\system32> Get-Childitem cert:\LocalMachine\root -Recurse | Where-Object {$.Issuer -ne $.Subject}
Try to move them to CA
PS C:\WINDOWS\system32> Get-Childitem cert:\LocalMachine\root -Recurse | Where-Object {$.Issuer -ne $.Subject} | Move- Item -Destination Cert:\LocalMachine\CA
B) The self-signed certificate thumbprint is not properly installed so this can block from connecting to xconnect.
C) Type mmc and open your Certificate Store for 'Local Computer'. Right-click your Sitecore certificate > All Tasks > Manage Private Keys. Grant full control to both NETWORK SERVICE and IIS_IUSRS. Do it for both xp0.xconnect and xp0.xconnect_client certificates.
D) In IIS Manager check the certificate is set for xConnect websites. Set the correct certificate from the list for xconnect website.

E) There might be thumprint value mismatched, please check xconnect and sitecore instance website certificate thumbprint value, try to place the correct thumbprint value in the intepub xconnect website App_Config folder appsetting.config , connectionstring.config and also in the sitecore app_config folder's config files . In case you have created a new certificate and their old certificate thumbprint values are present in that result it can show you an unavailable exception.
<add key="validateCertificateThumbprint" value="[CORRECT THUMBPRINT VALUE]" />
<add key="AllowInvalidClientCertificates" value="true" />
<add name="xconnect.collection.certificate" connectionString="StoreName=My;StoreLocation=LocalMachine;FindType=FindByThumbprint;FindValue=[CORRECT THUMBPRINT VALUE]" />
F) Check in the IIS xconnect application pool the identity is set to 'NETWORK SERVICE'
also, the xconnect website SSL settings for the content of website or application
require SSL checkbox is set to true and client certified is accepted
G) If none of the above options could able to solve the issue then please have a look that if there is any internal/external network setting is applied which might be also blocked TSL/SSL secure channel communication.