What you need to do is to double-check the path and placement of your `config` into the `Layers.config` file. Look at the below example. I need to disable a config file named `training.config`. And this is in the folder `\App_Config\Include\zzz`. So I added the location and the path in the `Layers.config` file like this. <layer name="Custom" includeFolder="/App_Config/Include/"> <loadOrder> <add path="Foundation" type="Folder" /> <add path="Feature" type="Folder" /> <add path="Project" type="Folder" /> <add path="/zzz/training.config" type="File" mode="off" /> </loadOrder> </layer> So you can see that I used the location under the include folder i.e. `/App_Config/Include/` and then given the path of the file under the `zzz` folder like this `/zzz/training.config` And this is working for me. Please check and verify the path and the location where you are adding in the `Layers.config` and try again. Hope this will solve your issue. Thanks